See the BD FACSDiva Software Reference Manual and the BD Cytometer Setup and Tracking Application Guide for more details.īD FACSDiva™ Software provides a graphic of the cytometer's physical optical configuration, showing the layout of detectors, filters and mirrors. Note that because of a change in the beads for BD FACSDiva™ Software v7.0 and v8.0, upgrading from BD FACSDiva™ Software v6.x to v8.0 will cause a loss of existing baselines and application settings.Īll baselines, performance checks and application settings will have to be recreated, as needed. This automation reduces startup time to approximately five minutes and eliminates multiple error-prone and expensive data acquisitions and calculations. Key cytometer setup values for PMT voltages, laser delay and area scaling factors are automatically calculated and adjusted to maintain optimal performance levels over time. PMTV, Qr, Br, Bright Bead Target Value, Bright Bead %rCV, Mid Bead Median, Mid Bead %rCV, Dim Bead Median, Dim Bead %rCV, Forward Scatter Laser Delay, Fluorescence Laser Delay, Laser Current, Laser Power, Fluidics Pressureĭefine Baseline, Check Performance, Auto Compensation, Reset Target ValuesĮxperiments, FCS Files, Statistics, Templates Left, Right, Bottom, Top, Distribute Horizontally, Distribute Vertically Histogram, 2D Dot Plot, 2D Contour Plot, 2D Density Plot Robust Standard Deviation, Robust Percent Coefficient of Variation Minimum, Maximum, Geometric Mean, Mean, Median, Standard Deviation, Percent Coefficient of Variation, Mode Interval, Rectangle, Polygon, Quadrants, Hinged QuadrantsĪuto-Polygon, Auto-Interval, Snap-To Polygon, Snap-To Interval User-Defined Event Number, User-Defined Percentage U, V and flat-bottom 96-well plates, flat-bottom 384-well plates, Terasaki plates (BD FACSAria™ Cytometer only)ġ45 (See Table 1 in Qr and Br in BD FACSDiva™ Software: Parameters for Characterizing Detector Performance)

BD FACSCanto™ 10-color, BD FACSCelesta™, BD FACSCanto™, BD FACSCanto™ II, BD FACSAria™ II, BD FACSAria™ III, BD FACSAria™ Fusion, BD ® LSR II, BD LSRFortessa™ and BD LSRFortessa™ X-20īD FACS™ Loader for the BD FACSCanto™ platform, BD ® High Throughput Sampler for the BD FACSCanto™ and BD ® LSR platforms, and BD FACS™ Accudrop for the BD FACSAria™ platform

Flowjo 10 save layout